The search for foods that do not use chemical preservatives in their composition has been growing over the years, and as a result, studies on active packaging have been intensified, which has as principle, in addition to protecting, transporting and containing the food, interact with it. In this context, the present study has developed an active antimicrobial, aromatic and biodegradable packaging in order to reduce the natural contamination of sliced Mozzarella cheese, which has shelf life of five days after manipulation. For this, a film was developed containing cassava starch, a plasticizing agent, gum arabic and oregano essential oil, as an antimicrobial agent. The research used the serial dilution methodology for counting and to aid in the isolation of the product natural contamination. The diffusion methodology in Agar in PDA medium with chloramphenicol was used to identify the antimicrobial power of the polymer matrix and the Casting method for film manufacture. With the results, it was possible to identify that only the film with the addition of oregano essential oil showed fungicidal action when compared to the same film without it, forming an inhibition halo of about 25 mm against Hyphopichia burtonii yeast isolated from the product itself, when stored under conditions stipulated by the supplier (Styrofoam trays stored in conventional refrigerator).
G. A. M Pietrowski,. et al., “Evaluation of the microbiological quality of mozzarella cheese marketed in the city of ponta grossa, Paraná”, Rev. Bras. Tecnol. Agroind. Paraná, vol. 2, no. 2, pp. 25 31, 2008.
S. Koelln, F. T. Mattana, E. Hermes, “Microbiological evaluation of mozzarella cheese and colonial cheese marketed in western Paraná.”, Rev. Bras. Tecnol. Agroind. Paraná, vol. 3, no. 2, pp. 66 74, 2009.
W.G.De Ruig and G. VandenBerg,”Inﬂuence of the fungicides sorbate and natamycin in cheese coatings on the quality of the cheese”, Neth. Milk Dairy J., vol. 39, pp. 165–172, 1985.
N. Y. Farkye, L. J. Kiely, R. D. Allshouse, and P. S., “Kindstedt., Proteolysis in Mozzarella cheese during refrigerated storage”., J. Dairy Sci, vol. 74, pp. 1433–1438, 1991.
P. S. Kindstedt, “Effect of manufacturing factors, composition and proteolysis on the functional characteristics of Mozzarella cheese.”, Crit. Rev. Food Sci. Nutr., vol. 33, pp. 167–187, 1993.
, A. L. Brody, “Say cheese and package it, please!”, Food Technol., vol. 55, pp. 76–77, 2001.
H. A. Diefes, S. S Rizvi and J. A. Bartsch, “Rheological behavior of frozen and thawed low-moisture, part-skim Mozzarella cheese”, J. Food Sci., vol. 58, pp. 764–769, 1993.
M. I. Kuo and S. Gunasekaran, “Effect of frozen storage on physical properties of pastaﬁlata and non pasta ﬁlata Mozzarella cheeses.”, J. Dairy Sci., vol. 86, pp. 1108–1117, 2003.
P. Appendini and J. H. Hotchkiss, “Review of antimicrobial food packaging”, Innovative Food Science & Emerging Technologies, vol. 3, pp. 113 126, 2002.
L. Bastarrachea, S. Dhawan; "S. Sablani, “Engineering Properties of Polymeric Based Antimicrobial Films for Food Packaging.” Food Engineering, vol. 3, pp. 90 93, 2011.
S. Gemili, Yemeniciog, A. ̆lu, S. A. Altınkaya, “Development of cellulose acetate based antimicrobial food packaging materials for controlled release of lysozyme.” Journal of Food Engineering, vol. 90, pp. 453 462, 2009.
C. Harvala, P. Menounos, N. Argyriadou, Medicinal Plants, 1987 Available in: <http://www.abq.org.br/cbq/2015/index.htmL>. Access in: 02/05/2019.
D. Vokou, S. Kokkini, J. M. Bessiere, “Geographic variation of Greek Oregano (Origanum vulgare ssp. hirtum) essential oils.”, Biochem. Syst. Ecol., vol. 21, pp. 287-295, 1993.
S. Kokkini, R. Karaousou, D. Vokou, Biochemistry System Ecologic, vol. 22, pp. 517, 1994.
W. Azambuja, Chemistry of essential oils and CAS, (2015). Available in: <http://www.oleosessenciais.org/quimica dos óleos essenciais e numero cas/> Access in: 02/05/2019.
E. D. R. Zavareze et al, “Development of oxidised and heat moisture treated potato starch fillm.”, Food Chemistry, vol. 132, no. 01, pp. 344 350, 2012.
Q.Yan et al., “Effects of extrusion and glycerol content on properties of oxidized and acetylated.”, Carbohydrate Polymers, vol. 87, pp. 707-712, 2012.
F. G. Torres et al. “Biodegradability and mechanical properties of starch films from Andean crops.” International Journal of Biological Macromolecules, vol. 48, pp. 603-609 , 2011.
I. Racowski, ”Evaluation of the antimicrobial activity of oregano essential oil in different concentrations against microorganisms present by natural contamination of sliced mozzarella cheese.” (2016) Available in: < https://microbiology.conferenceseries.com/2017/eposter/evaluation-of-theantimicrobial-activity-of-oregano-essential-oil-in-different-concentrations-against-microorganisms-present-by-natural-contamination-of-sliced-mozzarella-cheese-applied-microbiology-2017>. Access in: 09/05/2019.
C. Vanderzant, D. F. Splittstoesser. Compendium of methods for the microbiological examination of foods. Washington, DC: APHA, 2001, pp.1219.
A.M. L. de Moraes, R. de A. Paes, V. L. Holanda. Micologia. In: Concepts nd methods for the training of professionals in health laboratories. Volume 4. Rio de Janeiro: EPSJV, 2010, cap 4, p. 399-496. Available in: <http://www.epsjv.fiocruz .br/upload/d/cap4.pdf>. Access in: 27/04/2019. (2010).
N. R. Melo, Evaluation of Active Packaging by Incorporation of Nisin on the Inhibition of Staphylococcus sp. Dissertação Mestrado em Ciência e Tecnologia de Alimentos. Universidade Federal de Viçosa, Viçosa, 70p., 2003.
Everton, G. O.; Mandaji, C. M.; Sousa, F. T. N.; Teles, A. M.; Mouchrek, AN.; Costa, N. B. Microbiological evaluation of Mozzarella-type cheeses marketed in supermarkets in the city of São Luís- MA. In 55° Congresso Brasileiro de Química, São Paulo, Brasil, 2015.
O. Rosa, E. P. Carvalho, F. L. Dionízio, A. C. Ribeiro, “Indicators of unsatisfactory environmental contamination and hygienic processing conditions in minimally processed vegetables.” Higiene alimentar, vol. 18, no. 122, pp. 74-84, 2004.
J. A Arx,. J. P. W. Van der von, “The ascigerous state of Candida chodatii.”, Antonie van Leeuwenhoek, vol. 42, no. 3, pp. 309-314, 1976.
M. Groenewald, M. T. Smith, “Re-examination of strains formerly assigned to Hyphopichia burtonii, the phylogeny of the genus Hyphopichia, and the description of Hyphopichia pseudoburtonii sp.” International Journal of Systematic and Evolutionary Microbiology, vol. 60, pp. 2675-2680, 2010.
F. Todaro, D. Germano and G. Criseo, Mycopathologia. Retrieved from https://doi.org/10.1007/BF00437409 , 1983.
K. Guo et al. Intestinal Pichia burtonii associated with Qiweibaizhusan. (2013)Available in: <http://www.ijpsi.org/Papers/Vol2(9)/E029026033.pdf>. Access in: 02/05/2019.
S. K. Nayak, Biology of eukaryotic probiotics. M.T. Liong, ed. Probiotic biology, genetics and health aspects. Springer, Berlin, Heidelberg, 2011.
R. M. F. Magalhães, Antimicrobial activity of essential oils and incorporation in cellulose acetate film in the preservation of chilled chicken meat, (2012) Available in: <https://ppgcta.agro.ufg.br/up/71/o/RENATA_MARTINS_FERREIRA_MAGALHÃES_-_Dissertação_2012.pdf>. Access in: 08/03/2019.
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