Glycine max (L.) Merrill is a crop that brings a lot of economic and nutritive values, however soybean is quite sensitive to stress. The applications of gene technology can improve resistance of soybean plants against external stress factors. The aim of this study was to conduct a transgenic vector containing GmDREB6 gene and determine the expression of gene encoding GmDREB6 in Nicotiana tabacum before transforming into soybean plants. The GmDREB6 artificial gene was synthesized containing nucleotide fragment encoding 230 amino acids, nucleotide fragment encoding cmyc antigen and nucleotide fragments with cut-off points of XbaI/SacI enzyme pair. The results indicated that the 35S-GmDREB6-cmyc construct was designed and transferred into tobacco plants. The GmDREB6 was incorporated in the genome and was expressed in transgenic tobacco plants at the transcriptional level. The transgenic vector pBI121_GmDREB6 well worked on the model tobacco plants. Therefore, it can be used for transferring into soybean plants to enhance soybean tolerance to abiotic stress.
Thanh Son LO, Hoang Duc LE, Vu Thanh Thanh NGUYEN, Hoang Ha CHU, Van Son LE, Hoang Mau CHU, “Overexpression of a soybean expansin gene, GmEXP1, improves drought tolerance in transgenic tobacco”, Turk J Bot, vol. 39, pp. 988-995, 2015; http://doi:10.3906/bot-1502-40.
Dao Xuan Tan, Ho Manh Tuong, Vu Thi Thu Thuy, Le Van Son, and Chu Hoang Mau, “Cloning and Overexpression of GmDREB2 Gene from a Vietnamese Drought-resistant Soybean Variety”, Braz. Arch. Biol. Technol., vol 58, pp. 651-657, 2015; http://dx.doi.org/10.1590/S1516-89132015050170.
Lo Thi Mai Thu, Vi Thi Xuan Thuy, Duc Hoang Le, Le Le Son, Chu Hoang Ha, Chu Hoang Mau, “RNAi-mediated resistance to SMV and BYMV in transgenic tobacco”, Crop Breed. Appl. Biotechnol., vol.16, pp. 213-218, pp. 2016; http://dx.doi.org/10.1590/1984-70332016v16n3a32.
Hoang Thi Thao, Nguyen Thi Ngoc Lan, Ho Manh Tuong, Nguyen Vu Thanh Thanh, Le Van Son, Chu Hoang Mau, “Expression analysis of recombinant Vigna radiata plant defensin 1 protein in transgenic tobacco plants”, J App Biol Biotech, vol 5, pp. 070-075. https://doi:10.7324/JABB.2017.50112.
Thi Xuan Thuy Vi, Thi Ngoc Lan Nguyen, Thi Thanh Nhan Pham, Huu Quan Nguyen, Thi Hai Yen Nguyen, Quang Tan Tu, Van Son Le, Hoang Mau Chu, “Overexpression of the ZmDEF1 gene increases the resistance to weevil larvae in transgenic maize seeds”, Mol Biol Rep, vol. 46, pp. 2177–2185; https://doi.org/10.1007/s11033-019-04670-5.
Liu Y.W., Chen M., Xu Z.S., Zh G.Y., Li L.C., Ma Y.Z., “Glycine max dehydration-responsive element binding protein 6 mRNA, complete cds”. GenBank EF551166, 2007; https://www.ncbi.nlm.nih.gov/nuccore/EF551166.
Zhang X.X., Tang Y.J., Ma Q.B., Yang C.Y., Mu Y.H., Suo H.C., Luo L.H., Nian H. (2013) OsDREB2A, a Rice transcription factor, significantly affects salt tolerance in transgenic soybean. PLoS ONE 8:e83011, 2013; doi:83010.81371/journal.pone.0083011.
Topping J.F., “Tobacco transformation”, Methods Mol Biol., vol. 81, pp. 365–372, 1998; https://doi.org/10.1385/0-89603-385-6:365
Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A., Allard R.W., “Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics”, Proc Natl Acad Sci USA, vol. 81, pp. 8014–8018, 1984; doi: 10.1073/pnas.81.24.8014
Southern E.M., “Detection of specifc sequences among DNA fragments separated by gel electrophoresis”, J Mol Biol, vol. 98, pp.503–517, 1975; https://doi.org/10.1016/S0022-2836(75)80083-0
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